Webb• 5X T4 DNA Polymerase replacement synthesis buffer [165 mM Tris-acetate (pH 7.9), 50 mM magnesium acetate, 330 mM sodium acetate, 500 µg/ml nuclease-free BSA, 2.5 mM … WebbFor blunt ends or single base overhangs, incubate at 16°C overnight or room temperature for 2 hours (alternatively, high concentration T4 DNA Ligase can be used in a 10 minute ligation). Heat inactivate at 65°C for 10 minutes. Chill on ice and transform 1-5 μl of the reaction into 50 μl competent cells. Links to this resource Product Categories:

T4 DNA Ligase (5 U/µL)

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T4 DNA Polymerase - Thermo Fisher Scientific

WebbThe GC content and primer Tm are normal (within 40-60% and 58-68 degrees for Q5 High Fidelity Polymerase PCR respectively). I am confident the PCRs have worked as gel electrophoresis and sequencing... WebbThermo Scientific T4 RNA 连接酶催化寡核苷酸、单链 RNA 和 DNA 分子间和分子内 5'-磷酸基团和 3'-羟基末端间磷酸二酯键的 ATP 依赖性形成。 最少底物：分子间反应中为一个 … WebbThermo Fisher t4 rna ligase 1 T4 Rna Ligase 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … borja baston aston villa